A negative control slide is incubated with antibody diluent instead of primary antibody. The Catalyzed Signal Amplification System kit (Dako Cytomation, Carpinteria, CA, USA) and fluorescent IRDye 680 ...
Immunostaining with the two antibodies was carried out on 3-μm-thick tissue sections using ChemMate Envision/HRP (Polymer-Immuno Complex method, Dako Cytomation, Glostrup, Denmark). Micrographs ...
A negative control slide is incubated with antibody diluent instead of primary antibody. The Catalyzed Signal Amplification System kit (Dako Cytomation, Carpinteria, CA, USA) and fluorescent IRDye 680 ...
Membranes were washed in TBST then incubated with a 1/1,000 dilution of HRP-conjugated goat anti-mouse immunoglobulins (Dako Cytomation, Cambridgeshire, United Kingdom) in TBST for 1 h at room ...