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The first step in a northern blot is to denature, or separate, the RNA within the sample into single strands, which ensures that the strands are unfolded and that there is no bonding between strands.

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(Fragments greater than 1 kb long are not transferred efficiently by capillary action.) The gel is then soaked in alkaline solution to denature the DNA, and then neutralized in Tris/NaCl solution. The ...

Adjust the volumes to 9 μl with water. Denature the RNA by heating at 75 °C for 5 min, then rapidly chilling on ice. Total RNA extracted from cells with chaotropic agents is generally the ...

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It has many important parts, including: If body temperature increases over this temperature, enzymes will denature and become less effective at catalysing important reactions, such as respiration.

Again, the shape of the enzyme, along with its active site, will change. Extremes of pH also denature enzymes. The changes are usually permanent.

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