Nanoparticles were made by complex coacervation of chitosan and DNA as reported 17. Plasmid (10 μg) was added to 100 μl of sodium sulfate and heated to 55 °C. Chitosan (pH 5.7; 0.02% in a 25 mM ...
The microspheres are made using double emulsion, solvent extraction, or coacervation techniques. The presence of water/organic solvent interfaces in all of these methods results in protein ...