Summary and Introduction Clinical Application—Disease Entities Theoretical Framework for Therapy−The 'Steel Paradigm' Conclusion References Theoretical Framework for Therapy−The 'Steel ...

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The reaction system for ERIC-PCR consists of 16.25 μL of deionized water, 6.75 μL of RTaq DNA polymerase reaction mix (containing dATP, dTTP, dCTP, dGTP, etc.), 1 μL of ERIC2 primer (Table 1), and 1 ...

提高诱变率的其他方法 一般增加dATP和dTTP浓度时，宜选择含有Co2+的Buffer；而增加dCTP和dGTP 浓度时，宜选择含有Mn2+的Buffer。PCR反应缓冲液中的K+有利于扩增大于500 bp长度的DNA片段。 增加dTTP和dCTP浓度的致突变效果优于增加dATP和dGTP；使用5 mmol/L MgCl₂ 、0.5 mmol/L ...

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