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Science | 解旋酶介导的连续突变系统:实现哺乳动物细胞上的原位定向 ...
引言人类基因组中存在多达三十亿的碱基对,因此在基因组学的核心挑战和最终愿景,是描述该三十亿碱基对中,每一个碱基对于特定基因调控及下游蛋白质功能的影响。因此,如何系统且高通量地对目标基因组序列进行诱导突变,成为一项核心技术挑战。当研究者聚焦于单一基因组 ...
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